You can grab an icon using the Custom Region command, and you must save it using the .ico file extension. Icons must be exactly 32 X 32 pixels and no more than 16 colors. If an image has more than 16 colors, you may create a 16 color duplicate using the Create Duplicate command.
To capture an icon:
Choose Capture Image > Custom Region.
The Define Custom Region dialog box appears.
Under the Area Type heading, choose Icon.
Click Capture Area.
The Screen Rip32 window disappears and an icon-sized square appears on your screen.
Move the icon-sized square to surround the image you wish to grab.
Click the left mouse button.
The Screen Rip32 window reappears displaying the icon capture.