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- From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
- Newsgroups: bionet.software
- Subject: Re: Paperchase? How can I access it? (Boston/Beth Israel)
- Summary: Complete Information is in the FAQ list...
- Message-ID: <BxtMEK.Fs8@ncifcrf.gov>
- Date: 16 Nov 92 18:08:44 GMT
- References: <1992Nov15.201701.7068@ccu1.aukuni.ac.nz>
- Sender: Paul N. Hengen
- Organization: Frederick Cancer Research and Development Center
- Lines: 93
- Nntp-Posting-Host: fcsparc6.ncifcrf.gov
-
- nholford@ccu1.aukuni.ac.nz (Nick Holford) writes:
-
- >I have heard that there is a database of clinical trials under the name
- >Paperchase. It originates from Beth Israel Hospital clinical computing I
- >think. Can anyone tell me more about it and how I can access it?
-
-
- *******************************************************************
- * *
- * Frequently Asked Question (FAQ) list *
- * for bionet.molbio.methds-reagnts *
- * *
- * Last update was on 01 November 1992 *
- * *
- *******************************************************************
-
- This FAQ list is available by anonymous ftp to ncifcrf.gov
- Simply get the file pub/methods/FAQlist
-
- Paul N. Hengen
- National Cancer Institute
- Frederick Cancer Research and Development Center
- Frederick, Maryland 21702-1201 USA
-
- e-mail: pnh@ncifcrf.gov
-
- ================================================================================
- 1. What are some good reference books for methods in molecular biology?
- 2. Where can I get information about the genotypes and phenotypes of
- common E. coli strains used in molecular genetics?
- 3. How can I access MEDLINE?
- 4. How do I amplify pBR322 plasmid using chloramphenicol?
- 5. If a plasmid has more than one site for a particular restriction
- enzyme, is there some way to get the enzyme to cut at only one site?
- 6. How do I calibrate a Pipetman(tm) style micropipette?
- 7. Is it possible to get a personal copy of the files stored at Genbank?
- 8. How I do prepare powdered silica for DNA purification, with the
- associated solutions?
- 9. How do I use powdered silica to isolate DNA from agarose gels?
- 10. How do I use silica powder to prepare plasmid DNA for sequencing?
- 11. What is PCR?
- 12. What are some good reference books for PCR?
- 13. How should I select a set of primers to use for PCR?
- 14. What kinds of programs are available for designing PCR primers?
- 15. What is "Hot-start" PCR?
- 16. What is AP-PCR or RAPD PCR?
- 17. What is "Touchdown" PCR?
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- ---> How can I access MEDLINE? <---
-
- First, you'll need to get an account on MEDLARS. Call the MEDLARS Management
- Section Service Desk: 800-638-8480. Once you fill out the Online Billing
- Agreement with the NTIS, you get a userid and password from MEDLARS.
-
- Unless you're quite expert with MESH terms and MEDLINE searching, you should
- order a copy of GRATEFUL MED 6.0. You can then formulate your search offline
- and then telnet to medlars.nlm.nih.gov
-
- If you haven't got telnet, you can access MEDLINE via BITNIS and get your
- MEDLINE searches returned via email. You can do searches in command format or
- by using Grateful Med 6.9 and a program called smed.
-
- Some helpful books are:
-
- Albright, R. G. 1988. A basic guide to online information systems for
- health care professionals. Information Resource Press. Arlington, VA.
-
- Feinglos, S. J. 1985. MEDLINE: A basic guide to searching. Medical
- Library Association, Inc. Chicago, IL.
-
- You can access MEDLINE on the Internet through a service called the Life
- Science Network. This is a database service that offers access to over
- 80 life science databases, including MEDLINE. Although you will need a
- password and User ID to access the service, there is no charge to sign up
- and you're only charged for the information you retrieve -- no
- monthly minimum fees. To get a User Agreement, e-mail to:
-
- biosis@a1.relay.upenn.edu.
-
- You can also access Medline by way of Paperchase, a dial-up service available
- through a modem. They provide a 24 hr. toll-free number for questions.
- In the U.S. you can call 800-722-2075. From Canada, you can call
- collect 617-278-3900. You can also access Paperchase through
- telnet to biotechnet.com if you open an account with them first. The
- number for BioTechNET is 508-655-8282. You can get more information
- about this by sending e-mail to biotechnet@biotechnet.com.
-
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