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- Newsgroups: bionet.general
- Subject: Re: pUC119 : Info wanted.
- Message-ID: <robison1.722269706@husc10>
- From: robison1@husc10.harvard.edu (Keith Robison)
- Date: 20 Nov 92 14:28:26 GMT
- References: <28383@castle.ed.ac.uk> <19NOV92.16504495@wums.wustl.edu>
- Nntp-Posting-Host: husc10.harvard.edu
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-
- wetsel_r@wums.wustl.edu writes:
-
- >In a previous article, proberts@castle.ed.ac.uk (P Roberts) wrote:
- >>I'm looking for information on the plasmid pUC119. None of the
- >>databases to which I have access have heard of it. Does anyone out
- >>there have any information they could let me have? I would like to know
- >>things like marker (presumably ampicillin), polylinker and any other
- >>bits and bobs that it has. A complete sequence would be great if
- >>someone has a copy on-line that they could mail me.
- >> Thanks very much for any help you can offer.
- >>
- >> Paul (proberts@uk.ac.ed.castle)
- >----=
- >Paul:
-
- >Forgive the stupid question... are you sure it's pUC119 as in *119*????
- >I've heard of pUC19, as in *19*, but not 119. If it is in fact pUC19 that
- >you need, I think I can provide it to you but having been in mol-bio for 5
- >years, I've never heard of pUC119.
-
- I believe that you will find that pUC119 DOES exist -- it is pUC19 with
- an M13 ori added to convert it to a phagemid.
-
-
- Keith Robison
- Harvard University
- Department of Cellular & Developmental Biology
- Department of Genetics / HHMI
-
- robison@biosun.harvard.edu
-
-
-