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- Path: sparky!uunet!spool.mu.edu!agate!doc.ic.ac.uk!uknet!edcastle!proberts
- From: proberts@castle.ed.ac.uk (P Roberts)
- Newsgroups: bionet.general
- Subject: Re: pUC119 : Info wanted.
- Message-ID: <28453@castle.ed.ac.uk>
- Date: 20 Nov 92 11:56:19 GMT
- References: <28383@castle.ed.ac.uk> <19NOV92.16504495@wums.wustl.edu>
- Organization: Edinburgh University
- Lines: 24
-
- >> {request for info about pUC119 deleted}
-
- wetsel_r@wums.wustl.edu writes:
- >Forgive the stupid question... are you sure it's pUC119 as in *119*????
- >I've heard of pUC19, as in *19*, but not 119. If it is in fact pUC19 that
- >you need, I think I can provide it to you but having been in mol-bio for 5
- >years, I've never heard of pUC119.
-
- >Best regards,
-
- >David L. Haviland
- >haviland@kids.wustl.edu
-
- Yes, I am sure it is pUC119 and not pUC19. That is why I requested
- help. I too had never come across this particular plasmid despite
- having used various other pUC vectors (including pUC19). A kind person,
- whose name escapes me at the moment, has informed me via e-mail that
- pUC119 is a derivative of pUC19 that contains a phage origin of
- replication and suggested that I try some of the original Messing papers
- where they think it was mentioned. Many thanks for this information; I
- would, however, still be interested in acquiring the sequence if anyone
- has it on-line.
-
- Paul
-