The collection contains machines associated with the history of motoring. These are, in particular, motorcycles of domestic producers, a significant part is, however, represented by foreign products that had been driven on the territory of the Czech Republic in the past. The largest part of the Museum collection is represented by machines manufactured before the World War II.
About one hundred producers of motorcycles have operated on the territory of the Czech Republic in the past. The oldest period is represented in the collection by motorcycles of the factory Laurin & Klement from Mlada Bolestav that belonged to top-quality European products in the early 20th century and were also very successful in motorcycle racing. Historically the most important, as to the volume of production, were the brands JAWA and CZ which have been associated with the history of domestic motorcycle production since the 30s.
The permanent exhibition shows about 40 motorcycles, of which one half are of domestic production. However, an important part of the motorcycle collection of the National Technical Museum can also be found on the castle Kamen in South Bohemia where a permanent exhibition devoted to the history of domestic motorcycle production has been installed.