The oldest period of chemistry is represented in the collections by a complete alchemy laboratory. Its outfit and equipment are mostly replicas of vessels and tools from the late 15th century that were made in Glass Works Kavalier Sazava in 1914 or were made by Otakar Zachar in Kladno. The collection includes glass retorts, alembics, earthenware such as aludel, muffle, crucible, anvil, a primitive device for measuring furnace glow, distilling apparatus, iron tongs and pokers.
The whole collection, including furniture and mock-up furnaces, was handed over to the Office of President of the Republic and is included within a permanent exhibition in the Mihulka Tower at the Prague Castle where, together with artistic works, it documents the times of Rudolf II.