Column Histogram

Array Analyzer Menu: Objects > Column Histogram

This window shows in horizontal way a vertical column from a band or lane.

The statistical data for each blot is shown in the table. The vertical axis of the graphic shows the average pixel intensity value along each blot in the column (from 0 to 255). This value is the exact inversion of the image pixel values, so higher values mean darker pixels (in grayscale images 0 value represents black color and 255 is pure white)

Below the graphic the Rf line shows the relative position of each blot.

File name: fig11_bands01-rgb.png
Channel: Grayscale - Inverted

R.C

n

Mean

Min

Max

Coef.var.

Rf

01.01 1294 172.8126 88.0000 253.0000 36.2562 0.0833
02.01 1507 169.9859 88.0000 254.0000 34.5148 0.2130
03.01 1386 142.5499 88.0000 254.0000 40.6190 0.4537
04.01 936 123.4414 88.0000 235.0000 31.5136 0.5617
05.01 2452 189.6116 88.0000 254.0000 32.6437 0.8673