SLIDE 26
(Nancy Pratt)
So, at this point we knew it was time
for sperm. So here's the other end of the equipment. This is
all JoGayle's little mobile sperm lab set up here by the giraffe
enclosure, and she took this sperm out of the equitainer and warmed
it up and assessed it under the microscope, and then we - she
and Jennifer Buff, her technician, filled our syringes, and it
was a go. And we inseminated Shanthi - I should tell you we did
this twice. We did this two days apart, two different times with
two different samples from Onyx. The first day we inseminated
with 33 ml of semen - extended semen. It's yellow because of
an extender, which has egg yolk in it, and that helps keep it
alive and nourish it - it keeps it alive longer than it normally
would stay alive. So we inseminated with 33 ml of this mixture
to a total of about 8 billion live sperm, and on the second day
about 43 mls, and it was a, I think I have that backwards - 43
mls was the first day, and that was about 8 billion sperm, and
then 33 mls the second day, about 14 billion sperm. The second
day was a bit more concentrated.